Figure 1.
Representative chromatograms showing resolution of heteroduplex (peaks 1) from homoduplexes (peaks 2) created on reannealing PCR products of a male reference with males having the polymorphisms M4, M7, M9, M18, M19, and M20, respectively. Column—DNASep, 50 × 4.6-mm ID, Transgenomic, Inc.; linear gradient of 0.45% acetonitrile per minute in 0.1 m triethylammonium acetate, at pH 7.0, at a flow-rate of 0.9 ml/min; mobile phase temperatures: 52°C, 56°C, 54°C, 58°C, 58°C, and 57°C, for M4, M7, M9, M18, M19, and M20, respectively. PCR amplicons were injected directly into the preheated mobile phase.
