List of ESTs Exhibiting Significant (P < 0.01) Differences in Abundance in the HL60 Cell Line Induced by DMSO or TPA
| EST ID | HL60 | HL60 + TPA | HL60 + DMSO | Significance |
| 418 | 22 | 10 | 1 | 3 × 10−7 |
| 211 | 24 | 10 | 2 | 4 × 10−7 |
| 19 | 8 | 23 | 2 | 8 × 10−7 |
| 356 | 16 | 2 | 0 | 3 × 10−6 |
| 380 | 12 | 1 | 0 | 6 × 10−5 |
| 135 | 4 | 12 | 0 | 6 × 10−5 |
| 285 | 14 | 8 | 1 | 1 × 10−4 |
| 2015 | 0 | 11 | 0 | 2 × 10−4 |
| 244 | 0 | 1 | 14 | 3 × 10−4 |
| 293 | 13 | 6 | 1 | 3 × 10−4 |
| 292 | 11 | 0 | 1 | 5 × 10−4 |
| 650 | 14 | 5 | 2 | 5 × 10−4 |
| 335 | 15 | 3 | 3 | 9 × 10−4 |
| 444 | 10 | 4 | 1 | 2 × 10−3 |
| 1674 | 0 | 8 | 1 | 4 × 10−3 |
| 155 | 0 | 8 | 3 | 4 × 10−3 |
| 861 | 6 | 1 | 0 | 7 × 10−3 |
| 305 | 6 | 2 | 0 | 7 × 10−3 |
| 1806 | 0 | 6 | 0 | 7 × 10−3 |
| 1808 | 0 | 6 | 0 | 7 × 10−3 |
| 1766 | 0 | 6 | 0 | 7 × 10−3 |
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Only the probability (computed according to Equations and ) corresponding to the most significant transition (numbers in bold) is listed (Okubo et al. 1995). The total EST numbers sampled from the HL60, HL60 + TPA and HL60 + DMSO cDNA libraries are 845, 845, and 1058, respectively. ESTs 418, 211, 356, 285, 293, 292, 650, 335, 444, 861, 305 corresponding to ribosomal proteins, and EST 380, a tag to an unkown gene, exhibit a marked reduction of expression level in the DMSO- and/or TPA-induced differentiated states. In constrast, ESTs 135 (ferritin), 2015 (LD78/macrophage inflammatory protein), 1674 (methionine adenosyltransferase), 155 (thymosin β-4), 1806 (lipocortin), 1808 (thymosin β-10), and 1766 (a metallothionein) appear more abundant in the TPA-induced state, also highly enriched in EST 19 (the ubiquitous elongation factor 1-α). β-Actin (EST 244), is the only markedly increased tag in the DMSO-induced state. EST numbers, abundance data, and protein assignments are from the “body map” public expression data repository athttp://www.imcb.osaka-u.ac.jp (K. Okubo and K. Matsubara).
