The Largest Subunit of Human RNA Polymerase III Is Closely Related to the Largest Subunit of Yeast and Trypanosome RNA Polymerase III

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Figure 4.
Figure 4.

An antibody directed against hRPC155 immunoprecipitates an RNA polymerase III active in a nonspecific transcription assay. Immunoprecipitations were performed as detailed in Methods with preimmune or anti-hRPC155 (antibody α-CSH499, rabbit CS-377) antibodies and P11 fraction. Antibody-bound material was eluted with specific or nonspecific peptide and tested in a nonspecific transcription assay as described in Methods, in the absence or presence of 2 or 300 μg/ml of α-amanitin. The data are represented as total amounts of cpm retained on DE81 cellulose filters in the nonspecific transcription assay. Approximately 30% of the activity resistant to 2 μg/ml of α-amanitin was recoved in the specific immunoprecipitation.

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  1. Genome Res. 7: 1006-1019

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