Aap5cs and Aatps-1 contribute to the desiccation-tolerance in A. avenae. (A) Mortality rates of nematodes subjected to different desiccation times after RNAi targeting Aap5cs and Aatps-1. The dsgfp RNA was used as a control. The significance analysis was performed between the treatment group and the control group using a t-test. (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001. (B) After drying for 12 min, the nematodes with different treatments were incubated with the DCFH-DA probe to detect the level of ROS in the nematode's body. The red arrow indicates the dehydrated regions on the nematode. Bar = 20 µm. (C) Quantification of pixel intensity of ROS signal in A. avenae body. P-values were calculated by a two-sided Wilcoxon rank-sum test.
