Allelic imbalance in expression of secreted proteins. (A) Statistics of the number of allele expression differences among secreted protein with the same length but sequence variation at four ages: J2, J3/J4, female, and male. The significance of expression differences between replicates at different stages was calculated by t-test, and P < 0.05 was considered to be under allelic expression imbalance. (B) The top 50 secretory proteins with allelic imbalance expression at each developmental stage were used for heat map display, and no gene expression bias towards a certain haplotype was found. (C) Pfam domain analysis of secretory proteins with allelic imbalance expression revealed that they were mainly enriched in nematode feeding-related functional genes.
