Figure 4.
TT-TSS-seq protocol applied to RNA isolated from mESCs. (A) RNA electropherogram, as measured using TapeStation, showing the isolation of short RNAs for scaRNA-seq. The 25-nt peak represents the reference ladder. (B) Scheme of experimental set-up. (C) Density plots showing TSS locations detected using mESC poly(A)-TSS-seq, TT-TSS-seq, and scaRNA-seq (less than 300 nt) compared to annotated mouse TSSs. n = 2 biological repeats. (D) Dinucleotide frequencies analysis. The first nucleotide represents the −1 nucleotide, and the second nucleotide represents the identified +1 tag/TSS. A threshold of n = 3 counts was used.
