Hill–Shannon FIE-gene and functional family diversity in LUAD before and after duplication. (A) Hill–Shannon FIE-gene diversity significantly increased in LUAD tumors postduplication (95% CI, bootstrapped). Background-mutation-gene diversities did not show an equivalent postduplication increase (95% CI, bootstrapped). Log2–log2 plots compare pre- and postduplication FIE/mutation-gene diversity as a function of the number of FIEs/mutations, using rarefaction to estimate diversity at smaller sample sizes than observed (solid line) and extrapolation to estimate more than twice the observed number of FIEs/mutations sampled (dotted line). (B) Hill–Shannon FIE-FunFam diversity for FIEs derived using PDB structures. (C) Hill–Shannon FIE-FunFam diversity for FIEs derived using PDB structures and TED/AlphaFold models. (D) The long tail in the FIEs per gene distribution.
