A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest

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Figure 3.
Figure 3.

Generating a reporter cell line of the endogenous locus. (A) Using CRISPR-Cas9 to tag a gene of interest (GOI) with a fluorescent tag at the C or N terminus. (B) Following transfection of the CRISPR-Cas9 plasmids and selection of the tagged GOI cell line, GOI expression from its endogenous locus can be quantified by measuring fluorescent signal.

This Article

  1. Published in Advance February 17, 2026, doi: 10.1101/gr.281154.125 Genome Res. 36: 433-459

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