Polycomb misregulation in enterocytes drives tissue decline in the aging Drosophila intestine

S-phase histone genes are upregulated with global downregulation in aged guts. (A) RNAPIIS5P counts for the absolute difference (40-day-old – 15-day-old) counts versus average count (log₁₀(40-day-old + 15-day-old)/2) for all promoters in the Drosophila genome. (B) UCSC Genome Browser track of RNAPIIS5P at a representative histone gene repeat. Yellow boxes highlight the promoter region of each histone gene. (C) Bar charts depicting RNAPIIS5P counts per million (CPM) for each promoter of the histone gene repeat shown in B. Significance was assessed by a Poisson rate test compared to 15-day-old samples. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, and (****) P < 0.0001.