CTCF dynamics modulate cohesin positioning along the genome. (A) Illustration of simulated FRiP calculation: after recording extruder leg positions, the fraction that overlap CTCF barrier positions (highlighted in orange) is computed. (B) Heat map showing FRiP for CTCF site bound time (τb) versus occupancy. Experimental values (Hansen et al. 2017; Justice et al. 2020; Liu et al. 2021) depicted with dashed lines with values spanning 0.07–0.15. (C) FRiP as a function of τb at fixed occupancy. At small τb, FRiP increases rapidly before reaching a plateau. (D) FRiP as a function of occupancy at fixed τb. FRiP increases linearly with occupancy at high τb but remains low at all occupancies when τb ≪ τE (τE = 1312 sec). (E) FRiP before (blue) or after WAPL depletion (yellow) from Liu et al. (2021), alongside simulations with reference (blue) or higher (yellow) lifetime at the indicated barrier bound time (τb).
