Hi-C pile-up plots on single and pairwise CTCF sites show that loop extrusion by condensins in mitosis cannot be blocked by bound CTCF. (A–D) Aggregate of Hi-C signal binned at 10 kb in nonsynchronized mESCs on all interphase-bound CTCF sites (A), mitotic bookmarked sites (B), reduced CTCF sites (C), and CTCF sites that lose binding in mitosis (D). (E–H) Aggregate of Hi-C signal in mitotic mESCs on all interphase-bound CTCF sites (E), mitotic bookmarked sites (F), reduced CTCF sites (G), and CTCF sites that lose binding in mitosis (H). (I–P) Pile-up of Hi-C signal in 10-kb bins in nonsynchronized (I–L) and mitotic (M–P) mESCs of pairwise interactions within 250 kb at all interphase bound CTCF sites (I,M), bookmarked CTCF sites (J,N), reduced CTCF sites (K,O), and CTCF sites that lose binding in mitosis (L,P). All CTCF sites are plotted with respect to strand orientation of the motif.
