Coactivation of AR and GR influences a distinct population of single cells. (A) Uniform manifold approximation and projection (UMAP) of scRNA-seq from EtOH-treated (purple dots), Dex-treated (red dots), DHT-treated (blue dots), and DexDHT-treated (green dots) VCaP cells. (B) UMAP clustering from the scRNA-seq with subpopulations 0–15 (SP0–SP15) indicated with a distinct color and label. (C) UMAP of FKBP5 (top) and TMPRSS2 (lower) expression at the single-cell subpopulations. Intensity of the color depicts the mRNA expression (log1p normalized). (D) Dot plot of the expression of selected genes in the single-cell subpopulations. The size of the circle depicts the percentage of cells that express the gene. Intensity of the color depicts the average expression. (E) UMAP of gene expression at the single-cell subpopulations and genome browser tracks of chromatin binding of AR and GR for SPDEF (left) and ACSL3 (right). (F) Hallmark gene set pathway analysis of differentially expressed (DE) genes between SP8 and SP4 and between SP8+14 and SP4+13 in scRNA-seq data. Color scale represents −log10 P-value. All genome browser tracks are normalized to a total of 10 million reads.
