AR remodels chromatin accessibility thereby assisting the binding of GR. (A) Scatter plot represents the log2 FC of motif enrichment P-value between C1 and C2 sites. Distinct C1-preferred (light blue) and C2-preferred (black) motifs are indicated. Gray dashed lines represent ±0.5 log2 FC. (B) Percentage of GATA2, HOXB13, FOXA1, GRE, ARE, half ARE, PR, and NKX3-1 motif enrichment at the C1 (blue bars) and C2 (black bars) peaks. Statistical significance calculated with a chi-squared test. (C) Percentage of HRE-positive C1 and C2 sites with one (blue), two (red), three (yellow), four (green), or more than four (gray) ARE, GRE, PR, and half ARE motifs. (D) Venn diagrams depict the overlap the following: (top) C1 GRBs (blue circle) and antiandrogen-induced GRBs (ENZ-UP; black circle) and (bottom) C2 GRBs (red circle) and ENZ-UP (black circle) in VCaP cells. (E,F) Box plots represent the normalized log2 tag density of ATAC-seq (E) and SMARCA4 ChIP-seq (F) at the indicated sites. Statistical significance calculated using one-way ANOVA with a Bonferroni post hoc test. (G) Box plots represent the log2 FC of AR ChIP-seq (left) and ATAC-seq (right) in DHT-treated VCaP cells in the absence or presence of antiandrogen. Gray dashed lines represent the absence of change. Statistical significance calculated with an unpaired t-test. (H,I) Box plots represent the normalized log2 tag density of BRD4 ChIP-seq (H) and FOXA1 ChIP-seq (I) at the indicated sites. Statistical significance calculated using one-way ANOVA with a Bonferroni post hoc test. All box plots are normalized to a total of 10 million reads. (*) P < 0.05, (***) P < 0.001.
