Activation of AR assists the loading of GR to chromatin. (A) Venn diagrams depict the overlap of GR binding sites (GRBs) in Dex-treated (blue circle) and DexDHT-treated (red circle) VCaP cells. (B) Venn diagrams depict the overlap of AR binding sites (ARBs) in DHT-treated (orange circle) and DexDHT-treated (black circle) VCaP cells. (C) GR ChIP-seq, AR ChIP-seq, ATAC-seq, SMARCA4 ChIP-seq, BRD4 ChIP-seq, and FOXA1 ChIP-seq profiles at C1, C2, and C3 sites in VCaP cells. (C1) AR-assisted GRBs (DexDHT/Dex: FDR < 0.05, fold change [FC] > 2), (C2) stable GRBs, and (C3) AR-decreased GRBs (Dex/DexDHT: FDR < 0.05, FC > 2) upon the respective treatments. Each heatmap represents ±1 kb around the center of the GR peak. Binding intensity (tags per base pair per site) scale is noted below on a linear scale. (D) Genome browser track examples of AR, GR, SMARCA4, FOXA1, and BRD4 ChIP-seq and ATAC-seq at FKBP5 (left) and KLK3 (right) loci in VCaP cells. (E,F) Box plots represent the normalized log2 tag density of GR ChIP-seq (E) and AR ChIP-seq (F) at the indicated sites. Statistical significance calculated using one-way ANOVA with a Bonferroni post hoc test. All heatmaps, box plots, and genome browser tracks are normalized to a total of 10 million reads. (***) P < 0.001.
