Cell-type resolution map of exercise response enables differential analysis that reveals high differential expression and accessibility for fiber types and LUM+ FAP cells. (A) High-quality single-nuclei assay data were integrated and clustered with 14 cell types identified. For cell-type markers used for annotation, see Supplemental Figure S1B, and for cell-type proportion changes, see Supplemental Figure S2. (B) Enrichment analysis of biological processes in fiber types shows functionally different transcriptome profiles for each cell type. Color represents the percentage of upregulated genes in a given pathway, and larger dot radius denotes more significant biological function. For enrichment analysis for all cell types, see Supplemental Figure S4. For detailed description of differential analysis including statistical tests used, see Methods. (C,D) Volcano plots show the distributions of upregulated and downregulated genes (C) and chromatin regions (D) for each cell type. Each point denotes a differentially expressed gene (C) or differentially accessible region (D) for a given cell type. Cell types with no significant differential features are not shown. Circadian-regulated features are excluded and shown in Supplemental Figure S3A. (E,F) UpSet plots of differentially expressed genes (E) and differentially accessible regions (F). Cell types with fewer than 10 differential features are not included, and sets with one differential feature are not shown.
