Table 1.
Genes that affect mouth-form development in Pristionchus pacificus
| Ppa Gene | El Paco v2 annotation | Chr | Protein function | Mutant line | Mouth form (NGM) | Mouth form (S-medium) | References |
|---|---|---|---|---|---|---|---|
| Wild type | PS312/RS2333 | >90% Eu | >90% St | ||||
| eud-1 | PPA43535 | X | Sulfatase | tu445, tu442, tu450, RS3111 | 0% Eu | — | Ragsdale et al. 2013 |
| seud-1/sult-1 | PPA12547 | I | Sulfotransferase | iub7, iub8, tu1061, tu1232 | 100% Eu | 0% St | Bui et al. 2018; Namdeo et al. 2018 |
| nag-1 | PPA06134 | X | N-acetylglucosaminidase | tu1137, tu1142 | 100% Eu | 0% St | Sieriebriennikov et al. 2018 |
| nag-2 | PPA34489 | X | N-acetylglucosaminidase | tu1138, tu1143 | — | >90% St | Sieriebriennikov et al. 2018 |
| sul-2.2.1 | PPA06135 | X | Sulfatase | — | >90% Eu | 100% St | Ragsdale et al. 2013; Sieriebriennikov et al. 2018 |
| daf-12 | PPA28648a | I | Nuclear hormone receptor | tu381, tu389, tu390 | 85% Eub | — | Bento et al. 2010 |
| lsy-12 | ppa_stranded_DN30317_c2_g1_i3 | IV | Histone acetyltransferase | tu319 | 2% Eu | — | Serobyan et al. 2016 |
| mbd-2 | PPA09682 | V | Methyl-binding protein | tu365 | 0% Eu | — | Serobyan et al. 2016 |
| nhr-40 | PPA22002a | X | Nuclear hormone receptor | tu505, tu1421, tu1418, tu1419 | 0% Eu | 100% St | Kieninger et al. 2016; Sieriebriennikov et al. 2020 |
| nhr-1 | ppa_stranded_DN28508_c0_g1_i2 | X | Nuclear hormone receptor | tu515, tu1163, tu1164 | 0% Eu | — | Sieriebriennikov et al. 2020 |
| daf-19 | ppa_stranded_DN25985_c0_g1_i1 | II | Transcription factor | RS3011 (tu1035), RS3238 (tu1167) | 10% Eu | 100% St | Moreno et al. 2018, 2019 |
| daf-21 | ppa_stranded_DN30790_c0_g1_i2 | I | Heat-shock protein | tu519 | 52% Eu | — | Sieriebriennikov et al. 2017 |
| osm-1 | PPA24556a | I | Intraflagellar transport protein | RS3170 (tu1129) | 5% Eu | 100% St | Moreno et al. 2019 |
| osm-12 | PPA23445 | III | BBSome protein | tu1099, tu1414 | 100% Eu | 10% St | Moreno et al. 2019 |
| xbx-1 | PPA21711 | I | Dynein protein | RS3117 (tu1081) | 100% Eu | 70% St | Moreno et al. 2019 |
| ifta-1 | PPA23338 | II | Intraflagellar transport protein | RS2996 (tu1045) | 55% Eu | 97% St | Moreno et al. 2019 |
| dyf-1 | PPA06701 | V | Intraflagellar transport protein | RS3092 (tu1072) | 35% Eu | 98% St | Moreno et al. 2019 |
| che-11 | ppa_stranded_DN27945_c0_g1_i1 | II | Intraflagellar transport protein | RS3121 (tu1085) | 75% Eu | 98% St | Moreno et al. 2019 |
| che-3 | PPA11202 | V | Dynein protein | RS3141 (tu1100) | 33% Eu | 100% St | Moreno et al. 2019 |
| osm-3;klp-20 | PPA03370;a ppa_stranded_DN31699_c4_g13_i1 |
IV; III | Kinesin proteins | RS318 (tu1090; tu1131) | 33% Eu | 100% St | Moreno et al. 2019 |
| daf-25 | ppa_stranded_DN18164_c0g1_i1 | III | MYND domain protein | tu716 | Lenuzzi et al. 2023 | ||
| tax-2 | PPA07435 | V | Nucleotide-gated channel subunit | tu1291, tu1440 | >80% Eu | — | Lenuzzi et al. 2023 |
| daf-16 | PPA39986 | V | Transcription factor | tu1514, tu1515 | 43% Eu | — | Lenuzzi et al. 2023 |
| daf-11 | PPA14907 | I | Guanylyl cyclase | tu722, tu1438, tu1439, tu1440 | 80% Eu | — | Lenuzzi et al. 2023 |
| dpy-6 | PPA38488 | I | Mucin-type protein | RS3887 (tu743) | >90% Eu | — | Sun et al. 2022a |
| chs-2 | PPA39501 | II | Chitin synthase | RS3911(tu1651), RS3912 (tu1652) | Eu (toothless) | — | Sun et al. 2022b |
| daf-7.1;daf-7.2 | PPA36793; PPA27957 | III; X | TGF-β signaling ligand | RS4058 (tu1566; tu1573) | 60% Eu | — | Lo et al. 2022 |
| tag-68 | ppa_stranded_DN25910_c0_g2_i3a | V | I-Smad transcriptional regulator | RS3791 (tu1557), RS3792 (tu1558) | 52% Eu | — | Lo et al. 2022 |
| nas-6 | PPA30034 | IV | Astacin metalloprotease | RS3913 (tu1653) | 60% Eu | — | Ishita et al. 2023 |
| spr-5 | ppa_stranded_DN16305_c0_g1_i1 | V | H3K4me1/2 demethylase | cbh49, cbh81, cbh82, cbh149 | 50% Eu | — | Levis and Ragsdale 2023 |
| mdt-15.1 | ppa_stranded_DN24725_c0_g1_i8 | III | Transcriptional coregulatory | iub9, iub10, iub12 | Eu (aberrant) | — | Casasa et al. 2023 |
| pddl-1 | ppa_stranded_DN27845_c0_g3_i3c | IV | Delta-9 desaturase | RS4411 (tu2028), RS4412 (tu2029) | >100% Eue | — | Piskobulu et al. 2025 |
| pddl-3 | PPA40514 | IV | Delta-9 desaturase | RS4401 (tu2033), RS4402 (tu2034)d | >90% Eue | — | Piskobulu et al. 2025 |
| pddl-4 | ppa_stranded_DN27845_c0_g2_i1c | IV | Delta-9 desaturase | RS4398 (tu2030), RS4399 (tu2031)d | >90% Eue | — | Piskobulu et al. 2025 |
| dhs-28.1 | PPA20393 | X | Peroxisomal beta- oxidation | RS4147 (tu1855) | 100% Eue | — | Piskobulu et al. 2025 |
| daf-22.1;daf-22.2 | PPA39143;a PPA41516 | II; IV | Thiolase; thiolase | RS2770 (tu489; tu504) | 100% Eue | — | Markov et al. 2016; Piskobulu et al. 2025 |
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Gene IDs are based on the El Paco v2 gene annotation that was used for this transcriptomic analysis.
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aThese genes have different gene IDs in the updated gene annotation (El Paco version 3).
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bThis is our observed mouth-form frequency (Supplemental Fig. S18), which differs from the previously reported percent Eu.
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cThese genes are not annotated in El Paco version 2, so this is the El Paco version 3 annotation.
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dSee reference for additional mutant alleles of these genes.
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eMouth form of these mutants is less responsive to high glucose.
