
Transcriptional effects of mutations in the mouth-form GRN are temporally restricted. (A–K) Mean expression from DESeq2 normalized counts of mouth-form genes in wild-type PS312 and the indicated mutant line. (*) Time point with significant differences in expression (adjusted P-value < 0.05). (L) Mouth-form GRN with St-promoting genes colored in blue and Eu-promoting genes colored in red. Genes in black may regulate the development of both morphs. Solid lines represent gene ordering resulting from epistatic analysis, whereas dashed lines represent untested gene interactions. Gold lines represent new regulatory links inferred from the results of this study.











