ERBS groups are associated with distinct long-range chromatin looping features. (A) Aggregate Region Analysis of Hi-C data in MCF-7 cells shows how ERBS groups contribute to long-range chromatin interactions. (B) Percentage of ERBS involved in chromatin interactions from ESR1 ChIA-PET data (Encode, ENCSR000BZZ) (Fullwood et al. 2009). Error bars represent the standard error of the mean from three replicates. (C) Distribution of chromatin interaction distances involving ERBS. Dashed lines indicate median distances for each group. Significance of Wilcoxon test is indicated. (D) Number of loops per ERBS subgroup. Dashed lines represent subgroup means. Significance of Wilcoxon test is indicated. (E) Venn diagram showing overlap of genes looped by ERBS subclasses, based on ESR1 ChIA-PET data. Genes connected by at least two out of the three biological replicates were kept. (F) Snapshot of ERBS “hubs.” Green, blue, and yellow boxes represent induced, constitutive, and inactive ERBS, respectively. Colored arcs represent subgroup-specific loops identified by ESR1 ChIA-PET.
