ERBS subgroups display distinct chromatin landscapes. (A) Heat map (bottom) and average density profile (top) of ATAC-seq signal at ERBS in MCF-7 cells treated with DMSO (light) or E2 (dark) for 45 min (GSE117943) (Guan et al. 2019). (B) ATAC-seq signal snapshot at the TFF1 locus. Green, blue, and yellow boxes represent induced, constitutive, and inactive ERBS, respectively. Numbers represent signal intensity. (C) Pie chart depicting the genomic distribution of ERBS subgroups. (D) Left: Heat map of chromatin state emission parameters (E2-treated MCF-7), with rows as chromatin states and columns as histone marks. The color intensity reflects the coverage enrichment. Right: Distribution of ERBS subgroups across active (red) and repressive (blue) chromatin states. List of external data sets used is given in Supplemental Table 3. (E) Heat map of overlap between ERBS subgroups and 73 histone mark data sets (Supplemental Table 4). Active (red) and repressive (blue) histone marks are indicated. (F) Average density profiles for H3K4me1, H3K4me2, H3K4me3, and H3K27ac at ERBS in MCF-7 under vehicle, E2, or full-medium conditions. Green, blue, and yellow lines represent induced, constitutive, and inactive ERBS, respectively. List of external data sets used is given in Supplemental Table 5.
