A donor-specific, haploid assembly was created using Flye and HapDup (Methods). Colored bars indicate the position on the contig of functional V, D, J, and C gene segments. (A) The fully phased IGH locus, with the dashed lines depicting alternative structures in sections where the assembly differs structurally between the two haplotypes, such as the IGHV3-9 and IGHV1-8 structural variant. The D gene cluster is expanded to show that a block of D gene segments is missing from each haplotype. (B) The IGL locus, where the IGL C V-CLUSTER was assembled into a separate contig from the remaining V-CLUSTERs and the J-C-CLUSTERs. All gene segments were shared between both haplotypes.
