Evaluation of strategies for evidence-driven genome annotation using long-read RNA-seq

Performance analysis of gene prediction as a function of the number of reads. Sensitivity, precision (A), and number of missed loci (B) were obtained with different sample sizes of WTC11 cell line PacBio FLNC reads using evidence-based and evidence-driven approaches. Performance of the different genome annotation approaches with Illumina short-read and PacBio long-read technologies at the gene level. (C) Ab initio predictions. (D) Evidence-based models using PacBio and Illumina-assembled transcriptomes. (E) Evidence-driven approach with PacBio, Illumina reads or Illumina-assembled transcriptomes as the source of evidence for the prediction step.