Examples in which LiftOn improves the current T2T-CHM13 annotation. (A) In the NM_006065.5 transcript of the SIRPB1 gene, the current CHM13 annotation omits three coding exons. The LiftOn version finds those exons and increases the DNA sequence identity from 81% to 98%. (B) In transcript NM_001134939.1 of OAZ3, the CHM13 annotation incorporates a partial CDS in the second exon, leading to a truncated protein. LiftOn corrects this misannotation, increasing the protein sequence identity from 5.42% to 100%. (C) In transcript XM_047448259.1 of EPHA2, the published annotation chooses the wrong start codon. LiftOn finds a better start codon that improves the protein sequence identity from 2.4% to 98.7%. (D) In transcript NM_001099772.2 from CYP4B1, LiftOn shifts the donor site of the seventh coding exon by 11 nucleotides, fixing a frameshift and improving the protein sequence identity from 53% to 99%.
