Figure 3.
Deamination activity of APOBEC3B in vitro. (A) Cytosine deaminase activities of cell lysates from hGOiA3A and hGOiA3B lines following 3 µg/mL doxycycline treatment for 48 h. NC, negative control; rA3A, recombinant A3A. (B) Sequences in randomly selected 100 read pairs in recombinant APOBEC3B-exposed cell-free denatured genomic single stranded DNA for 60 sec. (C) Deamination rate of unmethylated cytosines in recombinant A3B-exposed cell-free DNA fragments. 0, negative control; 30, DNA exposed for 30 sec; 60, DNA exposed for 60 sec.
