
miR-216b is responsible for regulating the expression of tas1r3. (A) Volcano map of intestine miRNAs expression levels in grass carp before or after the FHT from carnivore to herbivore. (B) The miRNAs putative binding to tas1r3 3′ UTR. Gray means no significantly difference; red means significantly differentially expressed miRNAs of grass carp before and after the FHT. (C) The expression analysis of microRNA expression in intestine of grass carp before and after the FHT (n = 10). (D) Relative dual-luciferase activity analysis was performed to detect the effects of microRNA on tas1r3 3′ UTR activity (n = 3). (***) P < 0.001, (****) P < 0.0001.











