The performance of DeltaSplice and baseline methods in predicting ΔSSU of splice sites homologous to humans. (A) Distribution of ΔSSU between chimpanzee and human (top) and between mouse and human (bottom). In each panel, ΔSSU measured by RNA-seq is shown on the x-axis, and ΔSSU predicted by different methods is shown on the y-axis. DeltaSplice refers to the results obtained using the dual-sequence mode, and DeltaSplice (single) refers to the results obtained using the single-sequence mode. The retrained Pangolin and the original SpliceAI models were used. (B) Pearson's correlation between RNA-seq and predicted ΔSSU of splice sites homologous to humans using different methods. (C) Top k accuracy of different methods in predicting splice sites with |ΔSSU| ≥ 0.2, where k is the true number of exons showing the specified differences.
