Single-cell discovery of m⁶A RNA modifications in the hippocampus

Hippocampal single-cell identification following AAV transduction. (A) Schematic diagram of detecting m⁶A RNA modification in the mouse hippocampus on a single-cell level through AAV transduction with E-Yth and E-Yth^mut controls. (B) Integration of uniform manifold approximation and projection (UMAP) of 27,804 single-cell transcriptomes. Cluster numbers from zero to 27 are indicated. (C) Separate UMAP for E-YTH and E-YTH^mut: 11,561 single cells in E-YTH and 16,243 in E-YTH^mut. (Circle) Cluster 23 is missing in E-YTH. (D) Violin plot visualizing the number of genes per cluster. No genes were detected in cluster 23 in E-YTH samples. (Red) E-YTH, (blue) E-YTH^mut. (E) m⁶A counts per cluster. (F) Metagene analysis of individual cell clusters identified by single-cell sequencing. The m⁶A number surrounding the stop codon (position 0) is shown. m⁶A sites were obtained after eliminating background editing sites. Clusters 1, 18, 19, and 22 are shown, which represent different m⁶A distribution patterns.