Figure 1.
iTF-seq enables screening of numerous TFs with fate conversion potential. (A) Schematic representation of iTF-seq procedure and subsequent multi-omics approaches. (B) Representative western blot images showing inducible overexpression of biotinylated proteins in individual iTF lines detected by streptavidin-HRP. (*) Nonspecific bands, (Dox) doxycycline. (C) Morphology of 80 pooled iTF cell lines under uninduced condition (−Dox) and upon induction of TFs by treating Dox (0.5 μg/mL) for 1, 3, and 5 d. Scale bars, 100 μm or 200 μm (upper).
