P-element ping-pong is inactive in replicate 2. (A) The ping-pong signature (peak at position 10) of the P-element in ovaries at generation 35. Note that the ping-pong signature is missing in replicate 2. (B) The ping-pong signature of the P-element in replicate 2. Data are shown for all generations with sufficient piRNAs. Small RNA extracted from ovaries and whole flies is shown in dark and light gray, respectively. (C) Motifs of sense and antisense piRNAs (23–29 nt) complementary to the P-element in ovaries at generation 35. Sense piRNAs in replicate 2 do not show an A-bias at position 10. (D) Quasimodo displays a pronounced ping-pong signature in all replicates (ovaries, generation 35). (E) Motifs of sense and antisense piRNAs of Quasimodo (ovaries, generation 35).
