Figure 3.
pG4 lncRNA oligos show characteristic features of rG4s. (A) IDS showing normalized ΔA from 220 to 330 nm between unfolded (no K+) and folded (10 mM K+) conditions at 37°C. (B) TDS showing normalized ΔA from 220 to 330 nm between unfolded (95°C) and folded (37°C) in 10 mM K+ buffer. (C) CD spectra from 210 to 350 nm for G4 topology. (D) UV melt showing normalized absorbance at 260 nm from 90°C to 25°C, cooled at a rate of 1°C/min. Colored dashed lines show nonlinear fit of normalized data. Individual melt profiles (raw data) and Tm for each oligo tested are given in Supplemental Figure S10.
