Figure 6.
Nuclease-mediated fragmentation in knockout mice. (A) Pooled molecule size distributions of wild-type and nuclease-deficient mice from SMRT sequencing. Visualization of size in the range of 0 to 5000 bp and log10-transformed frequencies were used. (B) Zoom-in plot of A showing size in the range of 0 to 250 bp on the linear scale. (C) Boxplot showing the proportions of long molecules originated from 2000 bp upstream of and downstream from transcription start sites. (D) Percentage of changes in pooled long molecules (>500 bp) abundance relative to wild-type mice on low, medium, and high expression gene groups.
