Genomic origin, fragmentomics, and transcriptional properties of long cell-free DNA molecules in human plasma

Normalized end frequencies of SMRT sequencing data. (A) Normalized end frequencies of SMRT long and short molecules pooled from plasma of healthy individuals at TSSs of the expression-stratified gene groups EXP1 to EXP5, corresponding to low to high expression. Transcription start positions are denoted as position 0. All transcription start sites were strand-adjusted so that positive positions are in the direction of transcription. (B,C) Normalized end frequencies of SMRT long and short molecules pooled from plasma of healthy individuals at DHSs (B) and CTCF binding sites (C). DHSs or CTCF binding site peaks are denoted as position 0; downstream and upstream 2000 bp is shown.