The abundance of long cfDNA molecules for HCC detection. (A) Comparison of the abundance of SMRT sequencing molecules among healthy individuals, HBV carriers, and patients with HCC. The abundance of long and short molecules was measured using the top 5000 expressed genes in HCC tumor tissues. The Kruskal–Wallis test P-value for differences among groups. Post hoc pairwise Wilcoxon rank-sum test P-values with Benjamini–Hochberg adjustment are shown above horizontal lines. (B) ROCs of long molecule abundance measured in A for distinguishing individuals without HCC, including healthy subjects and HBV carriers, and with HCC. Multiple thresholds, including 0.1 million (total > 0.1M), 0.3 million (total > 0.3M), 0.5 million (total > 0.5M), and 1 million (total > 1M) molecules from a sample, were used to include samples for constructing ROCs.
