The abundance of long cfDNA molecules shows a positive correlation with transcriptional activity. (A,B) The abundance of long and short molecules on gene bodies of unexpressed and housekeeping genes for nonpregnant controls and pregnant subjects. (A) Data from SMRT sequencing. (B) Data from ONT sequencing. (C,D) The correlation between gene expression and molecule abundance. The median expression level of a gene across tissues was log-transformed and scaled. The median molecule abundance was derived from scaled expression levels. P-values by Pearson's correlation test. (C) Pooled data of 15 nonpregnant controls from SMRT sequencing. (D) Pooled data of 31 pregnant samples from ONT sequencing. (E,F) Comparisons of DNA methylation between long and short molecules. Data points from one sample are connected with a black line. P-values by Wilcoxon rank-sum test. (E) Data from SMRT sequencing. (F) Data from ONT sequencing.
