Distribution of long and short molecules from human plasma DNA. (A) Comparison of genomic representation on Chromosome 10 between long and short DNA molecules in 15 nonpregnant controls using SMRT sequencing. The overrepresentation and underrepresentation of long cfDNA molecules with respect to short molecules are indicated in blue and red, respectively. The genomic representation was determined based on 100-kb bins and was further smoothed by a 1-Mb moving average sliding window. The horizontal solid lines indicate normalized median differences between long and short molecules. The dashed rectangular boxes indicate one euchromatic (i) and one heterochromatic (ii) region. The track in between overrepresentation and underrepresentation of long molecules shows the chromosome ideogram. The ideogram band colors correspond to cytogenetic bands in UCSC Genome Browser. Darker bands are AT-rich, and lighter bands are GC-rich. Centromeric regions are indicated in dark green. The bottom track displays gene densities estimated by number of genes in 100-kb windows. (B) Comparison of genomic representation on Chromosome 10 between long and short DNA molecules in 31 pregnant samples using ONT sequencing.
