Combinations of two sgRNA selection tools select high-efficiency cleaving sgRNAs for several sgRNA efficiency screen data sets. Combinations of sgRNA selection tools in A are listed according to the overall cleavage efficiency of selected sgRNA significant (P < 0.05) for both methods (red) or one method (dark gray). Black indicates events with <5% of sgRNAs selected by at least one method. Comparison of sgRNA selection by different sgRNA selection tools shown as the median of the cleavage efficiency for individual data sets (B). The distribution of cleavage efficiencies for all sgRNAs is shown on the left (white box) and for PlatinumCRISPr–Wong score in red, Moreno-Mateos–Wong score in blue, and Azimuth–Xu score in green.
