LR-Ribo-STAMP can profile translation at the mRNA isoform level for cells in an unperturbed state. (A) Heatmap showing EditsC quantified in long-read APOBEC1-only and LR-Ribo-STAMP samples for two mRNA isoforms of the same gene. (B) LR-Ribo-STAMP EditsC and mRNA isoform expression for two isoforms, PCNP-201 (ENST00000265260, protein-coding) and PCNP-202 (ENST00000460231, NMD), of the gene PCNP. (C) Comparison of 5′-UTR length of highly versus lowly translated mRNA isoforms. (D) Comparison of highly translated 5′-UTR GC content versus lowly translated mRNA isoforms. (E) The contingency table used to analyze the differences in the proportion of isoforms having uORFs between highly and lowly translated isoforms. (F) Comparison of 3′-UTR length of highly versus lowly translated isoforms. (G,H) Comparative analysis of the different proportions of isoforms having miRNA binding sites (G) and RBP motifs in the 3′-UTR sequence (H) between highly versus lowly translated isoforms. Significance: (***) P ≤ 0.001, (**) P ≤ 0.01, (*) P ≤ 0.05.
