
Detection of extrachromosomal circular DNA (eccDNA) molecules containing Cast1, Cast2, Cast5, and Cast6 satDNA. Results of T. castaneum genomic DNA separation in 2D electrophoresis are shown on the left, and Southern blot hybridization with specific probes for each Cast element is shown on the right. Signals obtained by Southern blots confirm the presence of specific Cast satDNA in chromosomal linear double-stranded DNA (linDNA) but also in eccDNA molecules.











