An overview of the similarity between human and rhesus macaque DQ and DP genes and the members involved in the peptide loading pathway. On the top lane, a rhesus macaque MHC class II region spanning from DQA to DPB2 is illustrated at scale. This high-accuracy region configuration has been resolved using a combination of ONT and PacBio data (total coverage of >60×) for sequence similarity analysis. On bottom, the human reference genome (GRCh38.p14) is depicted for the equivalent region. The genes and their orientations are indicated by arrows. The color-coded bars connect homologous human and macaque genes and indicate their sequence similarity percentages that are determined using BLASTN (see Methods) (Altschul et al. 1990). The equivalents of HLA-DQA2 and -DQB2 are absent in macaques, as well as a homolog for HLA-DPA2. The remaining genes are shared in the two species, some with similarities up to 94%.
