Figure 3.
The full-transcript view offered by long-read sequencing allows for the observation of coordination between RNA variables. (A) When two alternative exons are coordinated, only molecules with either both exons included or excluded are observed in long-read sequencing data. (B) In TSS–exon coordination, an alternative exon may only be present in molecules using one of two alternative TSS. (C) In exon–poly(A) site coordination, an alternative exon may only be present in molecules using one of two alternative poly(A) sites.
