Challenges in identifying mRNA transcript starts and ends from long-read sequencing data

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Figure 2.
Figure 2.

Technical biases affecting the accuracy of terminal end sequences. (A) Multiple events during RNA handling and library preparation can introduce biases that influence the accuracy of terminal ends in LRS reads. (B) Nanopores present unique inherent hardware and software challenges that affect accurate sequencing, especially at the terminal ends of molecules.

This Article

  1. Genome Res. 34: 1719-1734

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