Box Figure 1.
Overview of current long-read RNA sequencing technologies. Depiction of RNA preparation, library preparation, and sequencing steps for ONT and PacBio technologies. On the left are shared RNA extraction and library preparation steps, in which reverse transcriptase is only required for the cDNA sequencing methods (both PacBio and ONT) and PCR is required for PacBio but optional for ONT. On the right are depictions of the varying adapter ligation and sequencing for ONT (top) and PacBio (bottom) methods.
