Table 2.
Summary of currently available adaptive sampling approaches
| Features | readfish | UNCALLED | ONT built-in | BOSS-RUNS |
|---|---|---|---|---|
| Instrument compatibility | MinION, GridION, PromethION | MinION, GridION | MinION, GridION, PromethION | MinION, GridION, PromethION |
| Number of targets | Enrichment: Min. 0.1%–Max. 10% of genome size for targets within same genome, 1%–5% optimal Depletion: Variable based on proportion in sample |
|||
| Customizable | ✓ | ✓ | ✓ | |
| Dynamic decisions | ✓ | ✓ | ||
| Setup | Requires GPU and considerable computational knowledge | Decent computational knowledge required | Not required | Requires GPU and considerable computational knowledge |
| Limitations | Nontrivial to install, requires reinstall for MinKNOW updates | Requires port codes for run initiation, nontrivial for GridION or PromethION | No dynamic decision capability | Nontrivial to install, requires reinstall for MinKNOW updates |
