Figure 4.
nCATS. DNA is dephosphorylated to prevent sequencing adapter ligation. Cas9 RNPs with guides specific to the ROI are used to cleave the DNA upstream and downstream from the targets. This exposes phosphate groups at the ends of the target strands to which sequencing adapters are then ligated. Targets are, therefore, preferentially sequenced from a sequencing pool consisting of adapter-bound targets and dephosphorylated nontarget DNA. (Created with BioRender; https://www.biorender.com/)
