Figure 3.
In-gel Cas9-cleavage and target-specific electrophoresis purification. Cells are embedded in agarose and lysed in gel, maintaining DNA fragment length. Cas9 cleavage is carried out in gel using guides specific to the ROI. PFGE is used to separate the target(s) from background DNA based on size, which is known a priori. The purified target is then prepared for sequencing on either of the long-read platforms using appropriate adapters/kits. (Created with BioRender; https://www.biorender.com/)
