Leveraging the power of long reads for targeted sequencing

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Figure 1.
Figure 1.

Long-range PCR enrichment. Primers are designed to flank ROI. PCR can be carried out as single reactions for single targets or with multiple targets in a single PCR reaction. Amplified targets can be optionally size-selected via gel if the target size is known. Amplicons are pooled together before library preparation (prep). (Created with BioRender; https://www.biorender.com/)

This Article

  1. Genome Res. 34: 1701-1718

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