Table 2.
Mutation frequency based on spectra
| Mutyh genotype | +/+ | −/− | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MF (×10−5) | ||||||||||
| KBrO3 (%) | 0 | 0.05 | 0.1 | 0.15 | 0.2 | 0 | 0.05 | 0.1 | 0.15 | 0.2 |
| Transition | ||||||||||
| G:C > A:T | 0.16 | 0.12 | 0.92 | 0.63 | − | 0.19 | 0.58 | 0.74 | 0.18 | − |
| A:T > G:C | − | 0.06 | 0.04 | 0.11 | 0.11 | 0.13 | − | 0.16 | 0.05 | − |
| Transversion | ||||||||||
| G:C > T:A | 0.08 | 0.31 | 0.34 | 0.28 | 0.47 | 0.16 | 1.02 | 2.25 | 2.54 | 2.73 |
| G:C > C:G | − | − | 0.06 | − | − | 0.04 | − | 0.03 | − | − |
| A:T > T:A | 0.05 | − | − | − | − | 0.1 | − | − | − | − |
| A:T > C:G | − | − | 0.03 | − | − | − | − | − | − | − |
| Indel | ||||||||||
| 1-bp del (poly(A)) |
0.49 (0.20) |
0.39 | 0.31 (0.06) |
0.7 (0.06) |
0.43 (0.14) |
0.57 (0.19) |
1.33 (0.06) |
0.48 (0.05) |
0.78 (0.06) |
0.49 |
| 1 bp ins (poly(A)) |
− | − | 0.02 (0.02) |
0.13 | − | − | − | 0.1 (0.1) |
0.1 (0.1) |
− − |
| >2-bp indel | 0.64 | 1.04 | 0.63 | 0.83 | 1.33 | 0.93 | 0.87 | 0.94 | 1.04 | 1.46 |
| Others | − | − | − | 0.1 | − | 0.05 | − | − | 0.07 | − |
| Total | 1.41 | 1.92 | 2.36a | 2.78a | 2.34 | 2.17a | 3.79 | 4.69a | 4.75a | 4.67 |
-
aTotal MF including more than two mutations detected in a single Km/Sm-resistant colonies. (−) Not detected.
