Cadmium (Cd) exposure screening in the mutant library. (A) The schematic diagram of abiotic stress screening in the mutant library with Cd exposure (left) and several scenes during the process (right). (B) The target genes survived from persistent Cd exposure. Individuals with sgRNA targeting KWMTBOMO12902 accounted for the highest proportion. (C) The expression level of KWMTBOMO12902 in midgut, fat body, and BmE cells with or without Cd exposure. (D) The survival rate of normal strain and KWMTBOMO12902-KO strain in Cd stress. The photographs were taken on the fifth day after Cd exposure. Only surviving silkworms were shown in the photograph, as the dead individuals were discarded to avoid cross-infection.
