Phenotypic characterization of the mutant library. (A) The body sizes, epidermal coloring, developmental duration, speckle patterns, appendage development, and metamorphosis arrest of 33 F1 lines. These 33 F1 strains showed significant morphological differences among the 300 F1 strains. (B) The development duration of different mutant lines. The genes that may be associated with development extending are highlighted by red; those in light blue are more possibly associated with a shortened development. (C) The whole cocoon weight of different mutant lines. The genes possibly associated with whole cocoon weight improvement are highlighted by red. Each blot represents the average cocoon weight of 50 individuals. (D) The cocoon shell ratio of different mutant lines. The genes possibly associated with cocoon shell ratio improvement are highlighted by red. Each blot represents the average cocoon shell ratio of 50 individuals. (E) The number of males and females in each line. The genes possibly associated with sex differentiation of silkworm are highlighted by red. (F) The short and long diameter of cocoon in each line. The genes that may regulate spinning behaviors are highlighted by red. Each blot represents the average diameters of 30 individuals.
