Effect of E-P genomic distance on transcriptional bursting kinetics. (A) A schematic diagram shows the increment of E-P genomic distance dG. (B) Influence of dG on burst size. Dashed green line represents the theoretical result, whereas circles show numerical simulations. The blue circles indicate significant differences compared with the end data point (red) via Kruskal–Wallis nonparametric test, and the gray circles indicate no significance. (C) Influence of dG on burst frequency, where the meanings of all symbols are the same as those in B. (D) Log–log plot shows the relationship between mean burst size and dG. Blue circles are the data points with significant differences in B. The red solid line indicates the power-law approximation (Equation 4) with the scaling exponent ℓBS = −0.16. Meanings of the symbols are also similar to those in B. (E) Log–log plot shows the relationship between burst frequency and dG. Meanings of the symbols are similar to those in D, but the scaling exponent is ℓBF = −0.19. (F) Log–log plot of experimental data from Zuin et al. (2022). Inset shows the log–log relationship between dG and encounter probability. The yellow line indicates an approximate power-law relationship for smaller dG.
