Ultra-long-range interactions (ULIs) between active promoters and distal regulatory elements. Pileup analysis. (A) Non-RNF2 overlapping CGIs split by quartiles based on CpG density (2352–3015 peaks per group). (B) Non-RNF2 overlapping TSSs (4800–5128 peaks per group) divided into quartiles by expression level, based on 4sU-seq data, in Micro-C data from mESCs. (C) The 10 kb surrounding the Q4 TSSs (5128 peaks) at 100-bp resolution in Micro-C data from mESCs. (D,E) CGI Q4 (2651 peaks) regions in Micro-C data from RAD21-AID mESCs (D) and Hi-C data from WT or Rnf2 KO mESCs (E). (F) TSS-distal H3K27ac peaks not overlapping RNF2 or CGIs split by quartiles based on H3K27ac ChIP-seq signal (6161–6452 peaks per group) in Micro-C data from mESCs. (G) CGI Q4 regions (2651 peaks; +CGI) and Q4 promoter-distal H3K27ac (6268 peaks; −CGI) in Micro-C data from mESCs.
